Applications for immunohistochemistry
Next to the regular antibody products, Monosan can offer you now Monosan Reagents. This product line contains a huge portfolio of detection systems for immunohistochemistry purposes like Monosan Plus (AP) Polymer Kits, Monosan Plus (HRP) Broad Spectrum Kits and Monosan (HRP) Broad Spectrum Kits. The Monosan Reagents detection kits are designed for the qualitative detection of antigens in fixed paraffin-embedded tissue sections, in frozen tissue sections, and in cytological samples. (The kits are developed for use in combination with mono- and polyclonal primary antibodies and sera obtained from mouse, rabbit, rat, and guinea pig.)
Beside the new detection systems MONOSAN® Reagents is offering you comprehensive accessories for the Immunohistochemistry:
* Dilution buffers
Example:
MONOSAN® Antibody Diluent is developed for dilution of primary antibodies.
Antibodies diluted with Antibody Diluent are primarily used in immunohistochemistry
with formalin-fixed paraffin-embedded tissue sections, but also with frozen, HOPE-fixed,
and cytological samples as well as in immunoblot procedures.
* Chromogens and Substrates
Example:
MONOSAN® Permanent AEC Kit is intended for immunohistochemical and in situ-hybridisation
staining procedures with horse radish peroxidase (HRP). AEC (3-Amino-9-ethylcarbazol)
leads to the formation of a red-brown precipitate at the location of the target
antigen or target nucleic acid. The precipitate is insoluble in aqueous mounting
media and can be observed by light microscopy.
* Histo Pens
* Pre-treatment solutions
Example:
MONOSAN® Fast Enzyme is a ready-to-use solution developed for enzymatic
epitope retrieval on formalin-fixed tissue sections on slides. This procedure (sometimes
called PIER, Protease Induced Epitope Retrieval) is used in immunohistochemical
staining procedures and prior to in situ hybridisations.
* Blocking reagents
Example:
The blocking solution MONOSAN® Peroxide Block is intended for inhibition
of endogenous peroxidase activity in tissue sections. It is primarily intended to
be used in immunohistochemistry on formalin-fixed paraffin-embedded samples if using
a detection system with horse radish peroxidase.
* Buffers
Example:
Monosan® HIER EDTA Buffer pH 8.0 is a solution developed for heat induced
epitope retrieval (HIER) in formalin-fixed paraffin-embedded tissue sections on
slides. This procedure is primarily used in immunohistochemistry.
* Miscellaneous