MONOSAN Reagents

Applications for immunohistochemistry (IHC). Next to the regular antibody products, MONOSAN® offers MONOSAN Reagents.

This product line contains a huge portfolio of detection systems for immunohistochemistry purposes like MONOSAN® Plus (AP) Polymer Kits and MONOSAN® (HRP) Broad Spectrum Kits. The MONOSAN® Reagents detection kits are designed for the qualitative detection of antigens in fixed paraffin-embedded tissue sections, in frozen tissue sections, and in cytological samples. (The kits are developed for use in combination with mono- and polyclonal primary antibodies and sera obtained from mouse, rabbit, rat, and guinea pig.)

Beside the new detection systems MONOSAN® Reagents is offering you comprehensive accessories for the Immunohistochemistry:

• Dilution buffers
  MONOSAN® Antibody Diluent is developed for dilution of primary antibodies. Antibodies diluted with Antibody Diluent are primarily used in immunohistochemistry with formalin-fixed paraffin-embedded tissue sections, but also with frozen, HOPE-fixed, and cytological samples as well as in immunoblot procedures.
  
  
• Chromogens and Substrates
  MONOSAN® Permanent AEC Kit is intended for immunohistochemical and in situ-hybridisation staining procedures with horse radish peroxidase (HRP). AEC (3-Amino-9-ethylcarbazol) leads to the formation of a red-brown precipitate at the location of the target antigen or target nucleic acid. The precipitate is insoluble in aqueous mounting media and can be observed by light microscopy.
• Histo Pens
  
  
• Pre-treatment solutions
  MONOSAN® Fast Enzyme is a ready-to-use solution developed for enzymatic epitope retrieval on formalin-fixed tissue sections on slides. This procedure (sometimes called PIER, Protease Induced Epitope Retrieval) is used in immunohistochemical staining procedures and prior to in situ hybridisations.
  
  
• Blocking reagents
  The blocking solution MONOSAN® Peroxide Block is intended for inhibition of endogenous peroxidase activity in tissue sections. It is primarily intended to be used in immunohistochemistry on formalin-fixed paraffin-embedded samples if using a detection system with horse radish peroxidase.
  
  
• Buffers
  MONOSAN® HIER EDTA Buffer pH 8.0 is a solution developed for Heat Induced Epitope Retrieval (HIER) in formalin-fixed paraffin-embedded tissue sections on slides. This procedure is primarily used in immunohistochemistry (IHC).